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Follicle Stimulating Hormone Human Recombinant
Product Name Follicle Stimulating Hormone Human Recombinant
Synonyms Follitropin subunit beta, Follicle-stimulating hormone beta subunit, FSH-beta, FSH-B, Follitropin beta chain, FSH.
Description FSH Human Recombinant produced in HEK-293 cells is heterodimeric, glycosylated, polypeptide chain transfected with two expression plasmids encoding the human FSH-alpha chain (Accession # P01215) (Ala25-Ser116) and human FSH-beta chain (Asn19-Glu129) (Accession # P01225) having a total Mw of 38kDa. FSH human recombinant is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P01225
Amino Acid SequenceFSH subunit alpha
Source HEK293
Physical Appearance and Stability Lyophilized recombinant FSH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FSH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity The recombinant FSH was lyophilized from a concentrated (1mg/ml) solution containing PBS. Greater than 95% as determined by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized Follicle Stimulating Hormone in sterile 18MO-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity The ED50 as determined by cAMP accumulation in human FSH Receptor transfected Chinese Hamster Ovary cells was found to be 80-450 pg/ml.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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