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Glial-Derived Neurotrophic Factor Mouse Recombinant
Product Name Glial-Derived Neurotrophic Factor Mouse Recombinant
Synonyms ATF1, ATF2, HFB1-GDNF, GDNF.
Description Glial derived Neurotrophic Factor Mouse Recombinant produced in E.Coli is a non-glycosylated homodimer containing 2 x 135 amino acids and having a total molecular mass of 30.2kDa. GDNF is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P48540
Amino Acid SequenceMSPDKQAAL PRRENRNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI.
Source Escherichia Coli.
Physical Appearance and Stability Lyophilized Glial-derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDNF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity GDNF was lyophilized with no additives. Greater than 98.0% as determined by analysis by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity The ED50 as determined by the dose-dependent proliferation of C6 cells, is 0.8-0.12µg/ml.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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