Goat anti-NOX5 Antibody |
| Principle Name | NOX5 |
| Official Symbol | NOX5 |
| All Name and Symbols | NOX5; NADPH oxidase, EF-hand calcium binding domain 5; MGC149776; MGC149777; NADPH oxidase 5; OTTHUMP00000164443; OTTHUMP00000164444 |
| Accession Number(s) | NP_078781.3; NP_001171708.1; NP_001171709.1 |
| Peptide Sequence | EWHPFTISSAPEQKD, is from internal region |
| Human Gene ID(s) | 79400 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize all reported isoforms (NP_078781.3; NP_001171708.1; NP_001171709.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Approx 90kDa band observed in Human Testis lysates (calculated MW of 86.4kDa according to NP_078781.3). Recommended concentration |
| Reference(s) | Choi H, Kim S, Kim HJ, Kim KM, Lee CH, Shin JH, Noh M, Sphingosylphosphorylcholine down-regulates filaggrin gene transcription through NOX5-based NADPH oxidase and cyclooxygenase-2 in human keratinocytes. Biochemical pharmacology 2010 Jul 80 (1) |