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| Name of Products | SV40-GFP Mouse Pulmonary Microvascular Endothelial Cells |
| Catalogue Number | cAP-m0003-SV40-GFP |
| Product Format | Frozen Vial |
| Cell Number | > 5x10[5] cells/vial |
| Mouse Pulmonary Microvascular Endothelial Cells (mPulMVECs) were isolated from lungs of 4-week old C57/Black6 mice and SV40-GFP-mPulMVECs were selected from mPulMVECs infected with SV40 and GFP expressing lentiviruses with puromycin. Mouse Endothelial Growth Medium (Low serum, cAP-69) is recommended for the expansion of SV40-GFP-mHtMVECs. And we warranty a minimum of 10 passages of the cells if cultured following the detailed protocol described below). |
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| 1. | Positive for PECAM-1. |
| 2. | Positive for VEGFR-2 |
| 3. | Can form tubular network on Matrigel |
| 4. | SV40-GFP-mPulMVECs are tested negative for common animal pathogen and mycoplasma. |
| The cells are offered for Research Use Only. |
| Frozen Vials in a Dry Ice Package. |
| When you receive the dry ice package with cells in frozen vials, transfer the frozen vials of cells into a -80C freezer for short period storage or into a liquid nitrogen tank for long-term storage. |
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| A) | Pre-coating of T25 flasks- Add 2ml each Quick Coating Solution (cAP-01) into a T25 flask to cover the whole surface of the flask, 5 mins later, dispose the excessive coating solution by aspiration and the flask is ready to be used. |
| B) | Thaw the frozen cell vial in a 37C water bath first, and then transfer the cells into the pre-coated T25 flask with 10ml of cAP-69 medium, cells usually become confluent overnight and ready to be passaged. |
| C) | To passage the cells, rinse the cells in a T25 flask with 5ml HBSS (RT) twice; then add 2ml Trypsin/EDTA (RT) (cAP-23) into one T25 flask; gently dispose the excessive Trypsin/EDTA solution within 60 seconds by aspiration. |
| D) | Leave the T25 flask with the cells at RT or 37C for 1 min (most cells usually will detach from the surface within 1-2 mins; or monitor the cells under a microscope until most of cells become rounded up, and then gently tap the flask against the bench surface, and the cells will move on the surface of the flask when monitoring under microscope. |
| E) | Add 5ml Trypsin Neutralization Buffer and spin down the cells with 800g centrifugation for 5 mins. |
| G) | Re-suspend the cell pellet with 15ml cAP-69 medium and transfer 5 ml each into 3 pre-coated T25 flasks (for 1/3 subculture ratio). |
| H) | Change medium every 2 or 3days and the cells usually become confluent within 7 days (when split at a 1/3 ratio). |
| The purchase of this product conveys to the buyer the nontransferable right to use the purchased amount of the product and all replicates and derivatives for research purposes conducted by the buyer in the buyer's laboratory only. |
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| Quick Coating Solution | cAP-01 |
| Mouse Endothelial Growth Medium (Low Serum) | cAP-69 |
| Endothelial Basal Medium | cAP-03 |
| HBSS w/o Ca2+, Mg2+ | cAP-11 |
| Cell Freezing Solution (FBS) | cAP-22 |
| Cell Freezing Solution (Non-FBS) | cAP-22B |
| Trypsin/EDTA Solution | cAP-23 |
| Trypsin Neutralization Solution | cAP-28 |
| ITS (100x) | cAP-26 |
| L-Glutamine-MAXIMUM (100x) | cAP-27 |
| Human Plasma Fibronectin Solution | cAP-42 |
| Handling human and animal tissue derived products is potentially bio-hazardous. Although each cell strain is tested negative for HIV, HBV and HCV DNA, or pathogens, diagnostic tests are not necessarily 100% accurate; therefore proper precautions must be taken to avoid inadvertent exposure. Always wear gloves and safety glasses when working with these materials. |
For customers using our primary cell products, we strongly advise you using our own medium, although similar cell growth medium can be purchased and used for our primary cell products. |
The quality of cells, in term of their growth features stated in our datasheets, will not be guaranteed if NON-ANGIO-PROTEOMIE's Cell Growth Medium is used. |
Due to the sensitive nature of primary cells and cell lines, all quality related issues about the cells products must be reported back to us within ONE month period after receiving the products, no quality warranty (i.e. replacement of cells) will be provided after the ONE-Month period. Thank you for your understanding.
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