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Goat anti-NCF1 / p47phox Antibody
Item#:
dAP-0027
Size Price Quantity
50.0 ug $310.00
Goat anti-NCF1 / p47phox Antibody
Principle Name NCF1 / p47phox
Official Symbol NCF1
All Name and Symbols NCF1; p47phox; FLJ79451; NCF1A; NOXO2; SH3PXD1A; neutrophil cytosolic factor 1; Neutrophil cytosolic factor-1 (47kD); neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1); NADPH oxidase organizer 2
Accession Number(s) NP_000256.3
Peptide Sequence SESTKRKLASAV, is from C Terminus
Human Gene ID(s) 653361
Non-Human GeneID(s) 17969 (mouse); 114553 (rat);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human, Mouse, Pig
Peptide ELISA antibody detection limit dilution 1 to 20000.
Western Blot Approx 48kDa band observed in lysates of cell line U937 and of Mouse Thymus, and 50kDa observed in Pig Spleen lysates (calculated MW of 44.7kDa according to NP_000256.3). Recommended concentration
Immunohistochemistry Paraffin embedded Human Colon, Neutrophils. Recommended concentration
Reference(s) Davidson-Moncada JK, Lopez-Lluch G, Segal AW, Dekker LV Involvement of protein kinase D in Fc gamma-receptor activation of the NADPH oxidase in neutrophils. Biochem J 2002 Apr 1;363(Pt 1)

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