Goat anti-ELMO1 Antibody |
| Principle Name | ELMO1 |
| Official Symbol | ELMO1 |
| All Name and Symbols | ELMO1; engulfment and cell motility 1 (ced-12 homolog, C. elegans); CED12; KIAA0281; ced-12 homolog 1; engulfment and cell motility 1; CED-12; ELMO-1; MGC126406; OTTHUMP00000207056; OTTHUMP00000207060 |
| Accession Number(s) | NP_055615.8; NP_569709.1; NP_001034548.1 |
| Peptide Sequence | PKEPSNYDFVYDCN, is from C Terminus |
| Human Gene ID(s) | 9844 |
| Non-Human GeneID(s) | 140580 (mouse); |
| Important Comments | This antibody is expected to recognise both human isoforms of this protein. NP_569709.1 and NP_001034548.1 represent the same isoform. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human, Mouse, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 83kDa band observed in Human Brain (Frontal Cortex) lysates, in Mouse and Rat Brain lysates and in lysates of cell line Jurkat (calculated MW of 83.8kDa according to NP_055615.8). Recommended concentration |
| Reference(s) | Gumienny TL, Brugnera E, Tosello-Trampont AC, Kinchen JM, Haney LB, Nishiwaki K, Walk SF, Nemergut ME, Macara IG, Francis R, Schedl T, Qin Y, Van Aelst L, Hengartner MO, Ravichandran KS. CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration. Cell. 2001 Oct 5;107(1) |
