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Goat anti-RNF34 / RFI (N Terminus) Antibody
Item#:
dAP-0179
Size Price Quantity
50.0 ug $310.00
Goat anti-RNF34 / RFI (N Terminus) Antibody
Principle Name RNF34 / RFI (N Terminus)
Official Symbol RNF34
All Name and Symbols RNF34; ring finger protein 34; RFI; RIF; RIFF; FLJ21786; RING finger protein RIFF; FYVE-RING finger protein MOMO; hRFI; CARP1; FLJ21786; caspases-8 and -10-associated RING finger protein 1
Accession Number(s) NP_919247.1; NP_079402.2
Peptide Sequence KAGATSMWASCC, is from N Terminus
Human Gene ID(s) 80196
Non-Human GeneID(s) 80751 (mouse); 282845 (rat);
Important Comments This antibody is expected to recognize both reported isoforms (NP_919247.1 and NP_079402.2).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot No signal obtained yet but low background observed in Human Testis lysate at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Immunohistochemistry In paraffin embedded Human Kidney shows cytoplasm staining in select DCT. Recommended concentration, 4-8µg/ml.
Reference(s) Sasaki S, Nakamura T, Arakawa H, Mori M, Watanabe T, Nagawa H, Croce CM. Isolation and characterization of a novel gene, hRFI, preferentially expressed in esophageal cancer. Oncogene. 2002 Jul 25;21(32)

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