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Goat anti-MURF2 / TRIM55 Antibody
Item#:
dAP-0187
Size Price Quantity
50.0 ug $310.00
Goat anti-MURF2 / TRIM55 Antibody
Principle Name MURF2 / TRIM55
Official Symbol TRIM55
All Name and Symbols TRIM55; RNF29; MURF-2; ring finger protein 29; for muscle specific ring finger 2; tripartite motif-containing 55; muscle specific ring finger 2
Accession Number(s) NP_908973.1; NP_908974.1; NP_908975.1
Peptide Sequence HIFSFSWLNSLNE, is from C Terminus
Human Gene ID(s) 84675
Non-Human GeneID(s) 381485 (mouse); 365751 (rat);
Important Comments This antibody is expected to recognize isoforms 1, 3 and 4 (NP_908973.1, NP_908974.1and NP_908975.1 resp.).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Approx 85kDa band observed in Human Heart lysates (calculated MW of 60.5kDa according to NP_908973.1). The observed molecular weight corresponds to earlier findings in literature with the same antibody (Willis et al, Circ Res. 2007 Mar 2;10
Immunohistochemistry Paraffin embedded Human Heart. Recommended concentration
Reference(s) Centner T, Yano J, Kimura E, McElhinny AS, Pelin K, Witt CC, Bang ML, Trombitas K, Granzier H, Gregorio CC, Sorimachi H, Labeit S. Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain. J Mol Biol. 2001 Mar 2;306(4)

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