Goat anti-REV1L Antibody |
| Principle Name | REV1L |
| Official Symbol | REV1 |
| All Name and Symbols | REV1; REV1L; REV1-like (yeast); REV1; REV1 protein; REV1 (yeast homolog)- like; REV1 homolog (S. cerevisiae); FLJ21523; MGC163283; MGC26225; REV1- like |
| Accession Number(s) | NP_057400.1; NP_001032961.1 |
| Peptide Sequence | VLQQTYGSTLKVT, is from C Terminus |
| Human Gene ID(s) | 51455 |
| Non-Human GeneID(s) | 56210 (mouse); |
| Important Comments | This antibody is expected to recognise both reported isoforms. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | No signal obtained yet but low background observed in MOLT-4 lysates at up to 1.5µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? |
| Immunohistochemistry | In paraffin embedded Human Testis shows nuclear staining in spermatogonia and spermatocytes. Recommended concentration, 4-6µg/ml. |
| Reference(s) | Masuda Y, Takahashi M, Tsunekuni N, Minami T, Sumii M, Miyagawa K, Kamiya K. Masuda Y, Takahashi M, Tsunekuni N, Minami T, Sumii M, Miyagawa K, Kamiya K. J Biol Chem. 2001 May 4;276(18) |
