Goat anti-HPK1 / MAP4K1 Antibody

Item#:

dAP-0318

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-HPK1 / MAP4K1 Antibody
Principle Name	HPK1 / MAP4K1
Official Symbol	MAP4K1
All Name and Symbols	MAP4K1; mitogen-activated protein kinase kinase kinase kinase 1; HPK1; hematopoietic progenitor kinase 1
Accession Number(s)	NP_001036065.1; NP_009112.1
Peptide Sequence	DVVDPDIFNRDPR, is from N Terminus
Human Gene ID(s)	11184
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise isoform 1 (NP_001036065.1) and isoform 2 (NP_009112.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 95-100kDa band observed in Jurkat (predicted MW of 96kDa according to NP_009112). Recommended for use at 0.5-2µg/ml.
Immunohistochemistry	Paraffin embedded Human Thymus. Recommended concentration
Reference(s)	Hu MC, Qiu WR, Wang X, Meyer CF, Tan TH. Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade. Genes Dev. 1996 Sep 15;10(18)