Goat anti-BRF2 Antibody |
Principle Name | BRF2 |
Official Symbol | BRF2 |
All Name and Symbols | BRF2; BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like; BRFU; FLJ11052; TFIIIB50; transcription factor IIB- related factor, TFIIIB50; RNA polymerase III transcription initiation factor BRFU; RNA polymerase III transcription i |
Accession Number(s) | NP_060780.2 |
Peptide Sequence | QAARQAATSVPNPP, is from C Terminus |
Human Gene ID(s) | 55290 |
Non-Human GeneID(s) | |
Important Comments | |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
Applications | Pep ELISA, WB |
Tested Species | Human |
Peptide ELISA | antibody detection limit dilution 1 to 16000. |
Western Blot | Approx 50kDa band observed in HeLa lysates (predicted MW of 48kDa according to NP_060780). Recommended for use at 1-3µg/ml. |
Reference(s) | Schramm L, Pendergrast PS, Sun Y, Hernandez N. Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters. Genes Dev. 2000 Oct 15;14(20) |