Goat anti-PDE5A Antibody |
| Principle Name | PDE5A |
| Official Symbol | PDE5A |
| All Name and Symbols | PDE5A; CN5A; PDE5; PDE5A1; CGB-PDE; phosphodiesterase 5A, cGMP-specific; cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase |
| Accession Number(s) | NP_001074.2; NP_246273.2; NP_236914.2; (NP_237223) |
| Peptide Sequence | LINGESGQAKRN, is from C Terminus |
| Human Gene ID(s) | 8654 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 100kDa band observed in Human Lung lysates (calculated MW of 100kDa according to isoform 1, NP_001074.2). In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95kDa is observed. This band is not observed in |
| Reference(s) | Morelli A, Filippi S, Mancina R, Luconi M, Vignozzi L, Marini M, Orlando C, Vannelli GB, Aversa A, Natali A, Forti G, Giorgi M, Jannini EA, Ledda F, Maggi M. Androgens regulate phosphodiesterase type 5 expression and functional activity in corpora cavernosa. Endocrinology. 2004 May;145(5) |
