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Goat anti-Pyruvate dehydrogenase / PDHX Antibody
Item#:
dAP-0631
Size Price Quantity
50.0 ug $310.00
Goat anti-Pyruvate dehydrogenase / PDHX Antibody
Principle Name Pyruvate dehydrogenase / PDHX
Official Symbol PDHX
All Name and Symbols PDHX; pyruvate dehydrogenase complex, component X; E3BP; OPDX; PDX1; proX; DLDBP; E3-binding protein; pyruvate dehydrogenase complex, lipoyl-containing component X; dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; pyruvate
Accession Number(s) NP_003468.2; NP_001128496.1; NP_001159630.1
Peptide Sequence KSFKANLENPIRLA, is from C Terminus
Human Gene ID(s) 8050
Non-Human GeneID(s) 27402 (mouse);
Important Comments This antibody is expected to recognise all reported isoforms.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 50kDa band observed in Human Pancreas lysate (calculated MW of 54.2kDa according to NP_003468.1). In transfected HEK293 transiently expressing PDHX a band of approx. 50kDa is observed. This band is not observed in the non-transfecte
Reference(s) Roche TE, Baker JC, Yan X, Hiromasa Y, Gong X, Peng T, Dong J, Turkan A, Kasten SA. Distinct regulatory properties of pyruvate dehydrogenase kinase and phosphatase isoforms Prog Nucleic Acid Res Mol Biol. 2001;70

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