Goat anti-RENT2 / UPF2 Antibody

Item#:

dAP-0775

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-RENT2 / UPF2 Antibody
Principle Name	RENT2 / UPF2
Official Symbol	UPF2
All Name and Symbols	UPF2; UPF2 regulator of nonsense transcripts homolog (yeast); HGNC
Accession Number(s)	NP_542166.1; NP_056357.1
Peptide Sequence	PRYQHPKGAPNAD, is from C Terminus
Human Gene ID(s)	26019
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise both reported isoforms (NP_542166.1; NP_056357.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 4000.
Western Blot	Preliminary experiments in Human HeLa lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibod
Reference(s)	Hosoda N, Kim YK, Lejeune F, Maquat LE. CBP80 promotes interaction of Upf1 with Upf2 during nonsense-mediated mRNA decay in mammalian cells. Nat Struct Mol Biol. 2005 Oct;12(10)