Goat anti-CD14 Antibody |
| Principle Name | CD14 |
| Official Symbol | CD14 |
| All Name and Symbols | CD14; CD14 antigen ; HGNC |
| Accession Number(s) | NP_000582.1 |
| Peptide Sequence | KRVDADADPRQYAD, is from internal region |
| Human Gene ID(s) | 929 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 55kDa band observed in Human Lymph Node lysates (calculated MW of 40.1kDa according to NP_000582.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 20 |
| Reference(s) | Iwaki D, Nishitani C, Mitsuzawa H, Hyakushima N, Sano H, Kuroki Y. The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain. Biochem Biophys Res Commun. 2005 Mar 4;328(1) |
