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Goat anti-ROBO1 / DUTT1 (Internal) Antibody


Item#:
dAP-0879
Size Price Quantity
50.0 ug $310.00
Goat anti-ROBO1 / DUTT1 (Internal) Antibody
Principle Name ROBO1 / DUTT1 (Internal)
Official Symbol ROBO1
All Name and Symbols ROBO1; DUTT1; roundabout, axon guidance receptor, homolog 1 (Drosophila) ; HGNC
Accession Number(s) NP_002932.1; NP_598334.1; NP_001139316.1; NP_001139317.1
Peptide Sequence GDVDLSNKINEMK, is from internal region
Human Gene ID(s) 6091
Non-Human GeneID(s) 19876 (mouse); 58946 (rat);
Important Comments This antibody is expected to recognise all reported isoforms (NP_002932.1; NP_598334.1; NP_001139316.1; NP_001139317.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 8000.
Western Blot Approx 250kDa band observed in Human Endothelial Cell line HUVEC lysates (calculated MW of 181kDa according to NP_002932.1). Some of the observed proteolytic breakdown products are also found in Human, Mouse and Rat Brain lysates. Recommen
Immunohistochemistry Paraffin embedded Human Liver. Recommended concentration
Reference(s) Hannula-Jouppi K, Kaminen-Ahola N, Taipale M, Eklund R, Nopola-Hemmi J, Kaariainen H, Kere J. The Axon Guidance Receptor Gene ROBO1 Is a Candidate Gene for Developmental Dyslexia. PLoS Genet. 2005 Oct;1(4)

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