Goat anti-GPR94 / TRA1 Antibody

Item#:

dAP-0881

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-GPR94 / TRA1 Antibody
Principle Name	GPR94 / TRA1
Official Symbol	HSP90B1
All Name and Symbols	TRA1; GRP94; tumor rejection antigen (gp96) 1 ; HGNC
Accession Number(s)	NP_003290.1
Peptide Sequence	KEGVKFDESEKTKE, is from internal region
Human Gene ID(s)	7184
Non-Human GeneID(s)	22027 (mouse); 362862 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human, Mouse
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 100kDa band observed in lysates of cell lines NIH3T3 (calculated MW of 92.5kDa according to NP_003290.1). Recommended concentration
Immunohistochemistry	In paraffin embedded Human Liver shows staining in some hepatocyte nuclei and weak cytoplasm staining of hepatocytes. Paraffin embedded Human Uterus. Recommended concentration
Reference(s)	Stojkovic M, Lako M, Stojkovic P, Stewart R, Przyborski S, Armstrong L, Evans J, Herbert M, Hyslop L, Ahmad S, Murdoch A, Strachan T. Derivation of human embryonic stem cells from day-8 blastocysts recovered after three-step in vitro culture. Stem Cells. 2004;22(5)