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Goat anti-AS160 / TBC1D4 Antibody
Item#:
dAP-0998
Size Price Quantity
50.0 ug $310.00
Goat anti-AS160 / TBC1D4 Antibody
Principle Name AS160 / TBC1D4
Official Symbol TBC1D4
All Name and Symbols TBC1D4; AS160; TBC1 domain family, member 4 ; RP11-159J2.1; DKFZp779C0666 ; Acrg embryonic lethality (mouse) minimal region ortholog; OTTHUMP00000040818; TBC (Tre-2, BUB2, CDC16) domain-containing protein; KIAA0603
Accession Number(s) NP_055647.2
Peptide Sequence DDPEKIEERKKSK, is from internal region
Human Gene ID(s) 9882
Non-Human GeneID(s)
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 150kDa band observed in lysates of cell lines Daudi and MOLT4 (calculated MW of 147kDa according to NP_055647.2). Recommended concentration
Immunohistochemistry In paraffin embedded Human Skeletal Muscle shows striated pattern staining of majority of muscular fibres. Recommended concentration, 3-5µg/ml.
Reference(s) Karlsson HK, Zierath JR, Kane S, Krook A, Lienhard GE, Wallberg-Henriksson H. Insulin-stimulated phosphorylation of the Akt substrate AS160 is impaired in skeletal muscle of type 2 diabetic subjects. Diabetes. 2005 Jun;54(6)

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