Goat anti-MARCH-IV Antibody

Item#:

dAP-1354

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-MARCH-IV Antibody
Principle Name	MARCH-IV
Official Symbol	MARCH4
All Name and Symbols	MARCH4; membrane-associated ring finger (C3HC4) 4; MARCH-IV; MGC104908; RNF174; membrane-associated RING-CH protein IV
Accession Number(s)	NP_065865.1
Peptide Sequence	DFCKEKTEDRYS, is from internal region
Human Gene ID(s)	57574
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 4000.
Western Blot	Preliminary experiments in Human Brain (Amygdala, Cerebellum, Cerebral Cortex, Frontal Cortex) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the
Reference(s)	Bartee E, Mansouri M, Hovey Nerenberg BT, Gouveia K, Fruh K. Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins. J Virol. 2004 Feb;78(3)