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Goat anti-MK5 / MAPKAPK5 Antibody
Item#:
dAP-2858
Size Price Quantity
50.0 ug $310.00
Goat anti-MK5 / MAPKAPK5 Antibody
Principle Name MK5 / MAPKAPK5
Official Symbol Mapkapk5
All Name and Symbols MAPKAPK5; mitogen-activated protein kinase-activated protein kinase 5; MAPKAP-K5; MK-5; MK5; PRAK; MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAPK-5; p38-regulated/activated protein kinase
Accession Number(s) NP_003659.2; NP_620777.1
Peptide Sequence STEALDNVLPSAQ, is from internal region
Human Gene ID(s) 8550
Non-Human GeneID(s) 17165 (mouse); 498183 (rat);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB Trf, IP, IHC
Tested Species Human, Mouse
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP; only the expected band was observed in the MK5 lane after EB11927 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemi
Immunohistochemistry In paraffin embedded Human Kidney shows brushborder staining in the PCT. Recommended concentration, 4-8µg/ml.
Reference(s) Yoshizuka N, Chen RM, Xu Z, Liao R, Hong L, Hu WY, Yu G, Han J, Chen L, Sun P. A novel function of p38-regulated/activated kinase in endothelial cell migration and tumor angiogenesis. Mol Cell Biol. 2012 Feb;32(3)

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