Goat anti-HOXB6 Antibody |
| Principle Name | HOXB6 |
| Official Symbol | HOXB6 |
| All Name and Symbols | HOXB6; homeobox B6; HOX2; HOX2B; HU-2; Hox-2.2; homeo box 2B; homeo box B6; homeobox protein Hox-2.2; homeobox protein Hox-2B; homeobox protein Hox-B6; homeobox protein Hu-2 |
| Accession Number(s) | NP_061825.2 |
| Peptide Sequence | EPRKSDCAQDKS, is from internal region |
| Human Gene ID(s) | 3216 |
| Non-Human GeneID(s) | 15414 (mouse); 497986 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 1000. |
| Western Blot | Approx 26kDa band observed in lysates of cell lines HEK293, CACO-2 and MRC5 PD19 (calculated MW of 25.4kDa according to NP_061825.2). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Lung shows nuclear staining in the alvioli. Recommended concentration, 5-10µg/ml. |
| Reference(s) | Ferrai C, Naum-Onganía G, Longobardi E, Palazzolo M, Disanza A, Diaz VM, Crippa MP, Scita G, Blasi F. Induction of HoxB transcription by retinoic acid requires actin polymerization. Mol Biol Cell. 2009 Aug;20(15) |
