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Goat anti-ERBB2 / HER2 Antibody
Item#:
dAP-3120
Size Price Quantity
50.0 ug $310.00
Goat anti-ERBB2 / HER2 Antibody
Principle Name ERBB2 / HER2
Official Symbol ERBB2
All Name and Symbols ERBB2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1; c-erb B2/neu protein; herstatin; metastatic lymph node gene 19 protein; neuroblas
Accession Number(s) NP_004439.2; NP_001005862.1
Peptide Sequence PREYVNARHCLP, is from internal region
Human Gene ID(s) 2064
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize both reported isoforms (NP_004439.2; NP_001005862.1). The immunizing peptide represents part of the extracellular domain.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Immunohistochemistry In paraffin embedded Human breast cancer shows cell membrane and cytoplasm staining in the affected cells of Her+ type and not in triple negative type. Recommended concentration, 4-6µg/ml.
Reference(s) Ahmad I, Patel R, Singh LB, Nixon C, Seywright M, Barnetson RJ, Brunton VG, Muller WJ, Edwards J, Sansom OJ, Leung HY. HER2 overcomes PTEN (loss)-induced senescence to cause aggressive prostate cancer. Proc Natl Acad Sci U S A. 2011 Sep 27;108(39)

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