Goat anti-CD96 Antibody |
Principle Name | CD96 |
Official Symbol | CD96 |
All Name and Symbols | CD96; CD96 molecule; TACTILE; CD96 antigen; T cell activation, increased late expression; T-cell surface protein tactile; cell surface antigen CD96; t cell-activated increased late expression protein |
Accession Number(s) | NP_937839.1; NP_005807.1 |
Peptide Sequence | KYTCIQEPNESD, is from C Terminus |
Human Gene ID(s) | 10225 |
Non-Human GeneID(s) | |
Important Comments | This antibody is expected to recognize both reported isoforms (NP_937839.1; NP_005807.1). |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
Applications | Pep ELISA |
Tested Species | |
Peptide ELISA | antibody detection limit dilution 1 to 128000. |
Western Blot | Preliminary experiments gave an approx 100+75kDa bands in Jurkat lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 65.6k |
Reference(s) | Hosen N, Park CY, Tatsumi N, Oji Y, Sugiyama H, Gramatzki M, Krensky AM, Weissman IL. CD96 is a leukemic stem cell-specific marker in human acute myeloid leukemia. Proceedings of the National Academy of Sciences of the United States of America 2007 Jun 104 (26) |