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Goat anti-PRKACA Antibody
Item#:
dAP-3329
Size Price Quantity
50.0 ug $310.00
Goat anti-PRKACA Antibody
Principle Name PRKACA
Official Symbol PRKACA
All Name and Symbols PRKACA; protein kinase, cAMP-dependent, catalytic, alpha; PKACA; PPNAD4; PKA C-alpha; cAMP-dependent protein kinase catalytic subunit alpha; protein kinase A catalytic subunit
Accession Number(s) NP_002721.1; NP_997401.1; NP_001291278.1
Peptide Sequence KHKETGNHYAMK, is from internal region
Human Gene ID(s) 5566
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize all three reported isoforms (NP_002721.1; NP_997401.1; NP_001291278.1). Based on the choice of the immunizing peptide there is no expected cross-reactivity with the other subunits.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Approx 40kDa band observed in lysates of cell line HeLa (calculated MW of 40.6kDa according to NP_997401.1). Recommended concentration
Reference(s) Goh G, Scholl UI, Healy JM, Choi M, Prasad ML, Nelson-Williams C, Kunstman JW, Kuntsman JW, Korah R, Suttorp AC, Dietrich D, Haase M, Willenberg HS, Stålberg P, Hellman P, Akerström G, Björklund P, Carling T, Lifton RP. Recurrent activating mutation in PRKACA in cortisol-producing adrenal tumors. Nature genetics 2014 Jun 46 (6)

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