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| Name of Products | hTert-Human Brain Microglia Cells (hTert-HBMgs) |
| Catalogue Number | cAP-0040-hTert |
| Product Format | Frozen Vial |
| Cell Number | >1x10[6]cells/vial |
| Microglia, one of the glial cell types in the CNS, is an important integral component of neuroglial cell network. They have been observed in the brain parenchyma from the early stage of development to the mature state. Microglia act as brain macrophages when programmed cell death occurs during brain development or when the CNS is injured or pathologically damaged. Microglia can be considered as the main cell in brain immune surveillance, can present antigens in the molecular context of MHC class II expression to CD-4 positive T cells, are capable of Fc mediated phagocytosis, and share many common antigens with hemopoietic and tissue macrophages. Furthermore, there is accumulating evidence that microglia are involved in a variety of physiological and pathological processes in the brain by interacting with neurons and other glial cells and through the production of biologically active substances such as growth factors, cytokines, and other factors. Human brain microglia cells (HBMgs, cAP-0040) are isolated from healthy human brain tissue. hTert-HBMgs have been cultured/passaged for more than 10 passages after infected with hTert-lentiviral particles under Special Formulated Microglial Cell Growth Medium (MgGM, cAP-37B). |
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| 1. | CD45 Positive |
| 2. | CD18 Positive |
| 3. | CD68 Positive |
| 5. | HBMgs are negative for HIV-1, HBV, HCV, and mycoplasma |
| Cells are offered for Research Use Only. |
| Frozen Vials in a Dry Ice Package. |
| When you receive the dry ice package with cells in frozen vials, transfer the frozen vials of cells into a -80C freezer for short period storage or a liquid nitrogen tank for long- term storage. |
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| A) | Prepare a Poly-L-Ornithine coated flask, Add 3 ml of Poly-L-Ornithine (10ug/ml in sterile H2O) into one T-25 flask and leave the flask @ room temperature for overnight. Matrigel can also be used for coating culture wares for microglial cells. |
| B) | Rinse the poly-L-Ornithine coated flask with sterile water twice and the flask is ready to be used. |
| C) | Warm MgGM (cAP-37B) before thawing the cells. |
| D) | Place the vial of the hTert-HBMs cells in a 37C water bath, hold and rotate the vial gently until the contents are completely thawed. |
| E) | Remove the vial from the water bath immediately, wipe it dry, rinse the vial with 70% ethanol and transfer it to a sterile field. Remove the cap, being careful not to touch the interior threads with fingers. Using 1 ml Eppendorf pipette gently resuspend the contents of the vial. |
| F) | Dispense the contents of the vial into 10ml of Universal Growth Medium (cAP-01B) and then spin down the cells @ 1000rpm for 10mins. |
| G) | Discard the supernatant and resuspend the cells with 5 ml of pre-warmed full medium (cAP-37B) and transfer the cells into the pre-coated flask. |
| H) | Culture the cells in a 37C incubator with 5% CO2. |
| I) | For best result, do not disturb the culture for at least 16 hours after the culture has been initiated. Change the growth medium the next day. |
| hTert-HBMgs do not adhere to the flask tightly and handle the flask with care to avoid disturbance of the cells. If more cells are in suspension, please collect the cells by spinning down the cells (@1000rpm, 10min) when performing medium changes |
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| Microglia Growth Medium (MgGM) | cAP-37B |
| Poly-L-Ornithine stock solution (10 x ) | cAP-38B |
| HBSS w/o Ca2+, Mg2+ | cAP-11 |
| Cell Freezing Solution (FBS) | cAP-22 |
| Cell Freezing Solution (Non-FBS) | cAP-22B |
| Trypsin/EDTA Solution | cAP-23 |
| Handling human tissue derived products is potentially bio-hazardous, despite tested negative for HIV, HBV and HCV DNA; nonetheless, proper precautions must be taken to avoid inadvertent exposure. |
For customers using our primary cell products, we strongly advise you using our own medium, although similar cell growth medium can be purchased and used for our primary cell products. |
The quality of cells, in term of their growth features stated in our datasheets, will not be guaranteed if NON-ANGIO-PROTEOMIE's Cell Growth Medium is used. |
Due to the sensitive nature of primary cells and cell lines, all quality related issues about the cells products must be reported back to us within ONE month period after receiving the products, no quality warranty (i.e. replacement of cells) will be provided after the ONE-Month period. Thank you for your understanding.
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